Scanning electron microscopy and materials science laboratory

Ref. Prof. Sergio Caputi

Dr. Tonino Traini
Dr. Donato Di Iorio
Dr. Francesco De Angelis

Phone: 0871.3554143


Equipment Available:

LEO 435 VP Scanning Electron Microscope

The scanning electron microscope (SEM) provides information on the microstructural features of surfaces and interfaces to an enlargement of approximately 150.000 X with a resolution power of a few millionths millimetres.

photo 1: Scanning Electron Microscope (SEM)


JEOL JEM 1010 Transmission Electron Microscope (TEM)

The TEM can enlarge a sample over 1.000.000 X with a resolution inferior to 1O Å., It is used in ultra-structural investigations of biological samples concerning the organisation of cellular cytoplasms and the study of cellular organelles, membranes and proteins.

photo 2: TEM image

 


LLOYD LR 30 K Machine for Universal Tests

This is a device used for dynamic traction, compression and cyclic loading tests on various kinds of samples.

photo 3: Universal testing machine

 

 




Types of anlyses made with the scanning electron microscopy LEO 435 WP:


Biomaterials microstructural analysis


002: Microstructure of a material
used in bone regeneration

 
003: Collagen membrane

004: Calcium sulfate crystals
   




Biomaterials/living tissues interface analysis


005: Microstructure of bone-to-implant interface.
 
006: Fibrin clot on titanium implant.
Two red cells are visible

 



Fractographic analisys on Implant components,
metal, ceramics and composites samples


007: fracture surface of a titanium screw
 
008: Titanium screw: it is possibile to observe a crack line between the first and second thread


009: internal threads of a fractured implant
   

 



Peri-implant vascular network analysis with an innovative technique


010: vascular network in peri implant bone
 
011: Osteon with a central vessel. It is possibile to observe several branches running from the Havers canal into Volkmann canal



Histomorphometric analysis of hard dental tissues


012: enamel prisms
 
013: dentinal tubules with Tomes' fibres


014: dentinal tubules
 
015: Rooth of reabsorbing deciduous tooth




Accuracy analysis of implant components


016: contact between screw threads and internal implant threads
   




Implant surface analysis


017: surface of a plasma spray implant
 
018: surface of an acid-etched implant



Cellular Morphology


019: human periodontal ligament derived by mesechymal stem cells
   



Ultra-structural studies on adhesion mechanisms


19A: scanning electron microscopy
image of a restored tooth

 
19B: adhesion to enamel

19C: adhesion to dentine
 
19D: adhesion to dentine

LEO Electron Microscopy Ltd.
19E: adhesion of resin to dentine
 
19F: Adhesion to dentine with resin tags


19G: Image of enamel-dentine-adhesive complex
   



SEM evaluation of the adhesive interface of ceramic
laminate veners cemented with mycrohybrid composite


19H: Collagen fibres observed under
trasmission electron microscope
 
19I: enlargement of the previous image



Types of analyses made with the JEOL JEM 1010 transmission electron microscope:


Collagen fibres observed under trasmission electron microscope
 
A small sectioned vessel with a red cell observed under trasmission electron microscope


Cell in contact with fibrin sponge
 
Cell in contact with a biomaterial




Types of analyses made with the LLOYD LR 30 K machine for universal tests:

Under development ...