Equipment Available:
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LEO 435 VP Scanning Electron Microscope
The scanning electron microscope (SEM) provides information on the microstructural features of surfaces and interfaces to an enlargement of approximately 150.000 X with a resolution power of a few millionths millimetres.
photo 1: Scanning Electron Microscope (SEM) |
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JEOL JEM 1010 Transmission Electron Microscope (TEM)
The TEM can enlarge a sample over 1.000.000 X with a resolution inferior to 1O Å., It is used in ultra-structural investigations of biological samples concerning the organisation of cellular cytoplasms and the study of cellular organelles, membranes and proteins.
photo 2: TEM image
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Types of anlyses made with the scanning electron microscopy LEO 435 WP:
Biomaterials microstructural analysis

002: Microstructure of a material
used in bone regeneration
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003: Collagen membrane |

004: Calcium sulfate crystals |
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Biomaterials/living tissues interface analysis

005: Microstructure of bone-to-implant interface. |
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006: Fibrin clot on titanium implant.
Two red cells are visible |
Fractographic analisys on Implant components, metal, ceramics and composites samples

007: fracture surface of a titanium screw |
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008: Titanium screw: it is possibile to observe a crack line between the first and second thread
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009: internal threads of a fractured implant |
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Peri-implant vascular network analysis with an innovative technique

010: vascular network in peri implant bone |
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011: Osteon with a central vessel. It is possibile to observe several branches running from the Havers canal into Volkmann canal |
Histomorphometric analysis of hard dental tissues

012: enamel prisms |
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013:
dentinal tubules with Tomes' fibres
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014: dentinal tubules |
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015: Rooth of reabsorbing deciduous tooth
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Accuracy analysis of implant components

016: contact between screw threads and internal implant threads |
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Implant surface analysis

017: surface of a plasma spray implant |
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018: surface of an acid-etched implant |
Cellular Morphology

019: human periodontal ligament derived by mesechymal stem cells |
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Ultra-structural studies on adhesion mechanisms

19A: scanning electron microscopy
image of a restored tooth
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19B: adhesion to enamel |

19C: adhesion to dentine |
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19D: adhesion to dentine
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19E: adhesion of resin to dentine |
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19F: Adhesion to dentine with resin tags
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19G: Image of enamel-dentine-adhesive complex
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SEM evaluation of the adhesive interface of ceramic
laminate veners cemented with mycrohybrid composite

19H: Collagen fibres observed under
trasmission electron microscope |
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19I: enlargement of the previous image |
Types of analyses made with the JEOL JEM 1010 transmission electron microscope:

Collagen fibres observed under trasmission electron microscope |
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A small sectioned vessel with a red cell observed under trasmission electron microscope |

Cell in contact with fibrin sponge |
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Cell in contact with a biomaterial |
Types of analyses made with the LLOYD LR 30 K machine for universal tests:
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