Biomorphology Laboratory

Ref. Dr. Oriana Trubiani

Phone: 0871.3554097 / 3554089

Analyses carried out in the Biomorphology Laboratory :

continual cell line and primary cells cultures, morphological analysis at confocal microscopy and immunohistochemical analyses in light microscopy, in confocal microscopy and transmission electron microscopy are carried out.

Biochemical analyses: mono-dimensional electrophoresis, immunoprecipitation, western blotting, RT- PCR.

Equipment available:

  • Laminar flow hood

  • CO2 incubator

  • Microcentrifuge

  • Refrigerated centrifuge

  • Biophotometer

  • Light microscopy

  • Inverted Light Microscopy

  • Termal cycler

  • Electrophoresys and western blot instruments



Main research areas:

Morphological, functional and molecular characterization of ex-vivo cultures of human stem mesenchymal cells from periodontal ligament and human dental pulp.

Verification studies on the biocompatibility of engignerized biological materials hosting human dental stem cells.


Stem cells seeded on fibrin sponge  
Scanning microscopy analysis of mesenchimal stem cells from periodontal ligament


In-vitro and in-vivo evaluation of the performance of mesenchimal stem cells from periodontal ligament and dental pulp on engignerized biomaterials and implant surfaces.



Stem cells cultured on fibrin sponge

 
Stem cells seeed on xenogenic biomaterials

Periodontal Ligament mesenchymal
stem cells at II° passage of culture

 
Periodontal Ligament mesenchymal
stem cells on MTA scaffold
PDL - MSCS cultured on titanic implant surface.
Note the intimate contact between cells and bioengignerized surface